Hello peoples,SURF is almost at it’s halfway point. It’s crazy that I’ve already been working for this long. Wooo. Okay, now to the stuff that happened.Something really sad happened last week. I’m not sure if you all remember, but a little while ago, I told you about a construct that I was making that had a Deformed promoter. Basically, I have been trying to clone in this promoter, but have been having a lot of trouble. Earlier this week I found out that the reason why it wasn’t working was because I was messing up on a crucial step. -__- . Oh well. Basically, in my notebook I read that we had to incubate the ligation, so the first thought that came into my head was in the 37 degree water bath. Well, it turns out that I was supposed to incubate at room temperature and that the ligase is inactivated in the water bath. So yeah, this is why my promoter wasn’t being ligated! I mean, it’s a pretty fail thing on my part, but at least I found this out now. I would hate losing more time.
Besides that, last week in lab went okay. I did some pretty basic stuff. Tuesday was kind of boring because I spent the entire day at the fly station. I started the morning off by seeing if the crosses I had set the week before had produced any transformants. For one fly line, this produced red-eyed flies, while transformants in the other fly line had orange eyes. I was able to find tons of red-eyed flies, but failed to find any orange eyed flies. Oh well. After looking for these transformants, I set up new crosses. It can be soooo boring sometimes! All you have to do is place either 3 mutant males or females with 3 wild-type males or females. After about 30 vials, it gets a little sickening. :) But, it had to be done and in a few days I’ll go check to see if we got any more transformants.
Almost a year ago now, I was just about to start my first Summer Undergraduate Research Fellowship (SURF) at JPL. NASA had sent out an email to all of their summer interns containing a social media template to announce that we had been selected as NASA interns. Excited to show my NASA pride, I posted it on my Instagram story, unaware of what would come out of this small action.
Hey hey! We’re starting a series where I walk you through my best finds for food and drinks in the Pasadena region, and in the LA metropolitan area. Diners, Drive-ins, and Dives, if you will (although, for copyright reasons we can’t call it that). As you explore your college options, I firmly believe that food and location are more important than your high school guidance counselor may lead you to believe. And I’m here to share my best finds from my time at Caltech with you.
Over the past several months, I have had the opportunity to intern at the NASA Jet Propulsion Laboratory (JPL) under the mentorship of senior research technologist Dr. Xiaoqing Pi. Dr. Pi’s guidance and mentorship has been instrumental to the development and success of my internship at JPL, where I use machine-learning to enhance the accuracy and integrity of navigation and communication signals. In addition to helping me develop an understanding of atmospheric and ionospheric remote sensing and machine-learning, Dr. Pi has often offered his insights on how to improve my researching skills. Dr. Pi was generous enough to take the time to answer a few questions regarding his research and advice for future student interns. I believe many students can benefit from some of the lessons that he has taught me:
The transition period to remote learning was a very uncertain time, especially for research and the Caltech Summer Undergraduate Research Fellowships (SURF) program. Many hands-on projects had to pivot at the last minute to facilitate off-campus contributions. However, many Techers were able to take advantage of the research opportunities offered at Caltech and JPL to make the best out of remote learning and research. To paint a picture, I’ve interviewed a few talented Techers for some insight on what researching from home looks like for them.